mRNA-LNP Characterization Services

Structural analysis of lipid nanoparticles for mRNA vaccine and therapeutic development. Understand your LNP organization, encapsulation efficiency, and batch-to-batch consistency.

What We Measure in Your LNPs

Beyond particle size: We analyze the internal structure of your lipid nanoparticles — not just the hydrodynamic diameter you get from DLS.

Lipid Bilayer Structure

  • Bilayer thickness and organization
  • Lipid phase identification
  • Ionizable lipid distribution
  • Lamellar vs. non-lamellar phases

Particle Architecture

  • Particle size distribution
  • Core-shell organization
  • PEG-lipid corona dimensions
  • Morphology determination

Encapsulation Assessment

  • mRNA/siRNA localization
  • Payload-lipid interaction
  • Internal electron density
  • Structural integrity

Supporting mRNA Therapeutic Development

From early formulation screening to batch release — structural characterization at every stage of your LNP development pipeline.

Formulation Development

Compare lipid compositions, ionizable lipid variants, and helper lipid ratios. Understand how formulation changes affect LNP structure.

Process Optimization

Characterize LNPs from different manufacturing parameters. Support scale-up with structural consistency data.

Stability Studies

Monitor structural changes during storage. Detect degradation, aggregation, or phase transitions over time.

Batch Release

Verify structural consistency for lot release. Compare production batches against reference standards.

Comparability Studies

Demonstrate structural equivalence after manufacturing changes. Support regulatory filings with comparability data.

Biosimilar Development

Structural comparison against reference products. Support regulatory submissions with detailed characterization.

Why Our LNP Analysis is Different

Internal Structure, Not Just Size

DLS tells you particle size. We reveal the internal organization — bilayer arrangement, core structure, and payload distribution.

Native Aqueous Conditions

Analyze your LNPs in their formulation buffer without staining, fixing, or drying. See the structure as it exists in your product.

Non-Destructive

X-ray scattering is non-destructive. Samples can be recovered for additional testing if needed.

Complementary to Cryo-TEM

We provide ensemble-averaged structural parameters that complement cryo-TEM imaging. Quantitative data from billions of particles.

Technical basis: Our analysis uses Small-Angle X-ray Scattering (SAXS) — an established technique for characterizing nanoscale structures in solution. Learn more about the methodology →

Structural Parameters We Determine

Size & Shape

  • Radius of gyration (Rg)
  • Particle size distribution
  • Shape determination (spherical, elongated)
  • Maximum dimension (Dmax)

Bilayer Characteristics

  • Bilayer thickness
  • Repeat spacing (multilamellar)
  • Lipid area per headgroup
  • Phase identification

Internal Organization

  • Core electron density
  • Shell/corona dimensions
  • Lamellar stacking
  • Internal structure models

Stability Indicators

  • Aggregation detection
  • Structural changes over time
  • Phase transition monitoring
  • Batch consistency verification

CMC & Regulatory Support

Structural characterization data formatted for regulatory submissions.

IND-Enabling Data

Early characterization supporting investigational filings. Establish structural acceptance criteria.

BLA/MAA Support

Comprehensive structural data for marketing applications. Batch release specifications.

Post-Approval Changes

Comparability studies supporting manufacturing changes. Site transfers and scale-up.

Documentation Standards

  • cGMP-compliant reports available for specific sample types (not always possible — please inquire)
  • 21 CFR Part 11 data integrity
  • QA-reviewed certificates
  • Method validation packages

Industrial Service Standards

Not academic research: We operate as an extension of your CMC laboratory — with committed timelines, confidentiality, and business terms.

Turnaround

Timelines discussed per project based on sample complexity and current capacity. We aim to meet your project deadlines where possible.

Complete Confidentiality

Your data is never published. NDAs accepted as-is. Strict sample handling protocols.

Since 2001

European laboratory with 20+ years pharmaceutical industry experience. Trusted by major pharma.

Example: Liposomal Drug Product Characterization

See how we characterized a liposomal formulation, determining bilayer structure, drug loading, and particle organization.

Liposome-Based Drug Product

Structural characterization of a liposomal formulation showing bilayer thickness, lamellarity, and drug encapsulation.

Read the case study →

Request LNP Characterization Services

Contact us to discuss your mRNA-LNP characterization needs. We'll recommend the appropriate analysis for your development stage.

Request Quote Learn About Our Methodology

Quick response: We typically respond to inquiries within one business day.
Sample requirements: Minimum 50 µL at ≥1 mg/mL lipid concentration (typical). We can discuss specific requirements for your samples.